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==Software==
*Mr Bayes for detailed instructions see the [http://mrbayes.csit.fsu.edu/wiki/index.php/Tutorial#Quick_Start_Version manual]
*Sequence Alignment Software (CLUSTALX or MUSCLE)
==Analysis==
#Generate sequence alignment and save as a nexus formatted file.
#Move file to Mr Bayes directory
#May need to edit the file to remove the line defining symbols.
#Type '''execute filename.exe''' with your filename specified
#Type '''lset nst=6 rates=invgamma'''
#Type '''mcmc ngen=10000 samplefreq=10'''. If the average standard deviation is below 0.01 then type no to continue. Else type yes until it reaches this threshold.
#Type '''sump burnin=250''', where 250 is 25% of your generations. For example if you ran 10 000 generations (sampled 1000 generations, you would use 250). Check that the potential scale reduction factor (PSRF) is close to 1.0 for all samples. If it is not run some more generations
#Type '''sump burnin=250''', where 250 is the same parameter as above. This will output both a cladogram and a tree file
#Transfer all files into another directory for saving.
*Mr Bayes for detailed instructions see the [http://mrbayes.csit.fsu.edu/wiki/index.php/Tutorial#Quick_Start_Version manual]
*Sequence Alignment Software (CLUSTALX or MUSCLE)
==Analysis==
#Generate sequence alignment and save as a nexus formatted file.
#Move file to Mr Bayes directory
#May need to edit the file to remove the line defining symbols.
#Type '''execute filename.exe''' with your filename specified
#Type '''lset nst=6 rates=invgamma'''
#Type '''mcmc ngen=10000 samplefreq=10'''. If the average standard deviation is below 0.01 then type no to continue. Else type yes until it reaches this threshold.
#Type '''sump burnin=250''', where 250 is 25% of your generations. For example if you ran 10 000 generations (sampled 1000 generations, you would use 250). Check that the potential scale reduction factor (PSRF) is close to 1.0 for all samples. If it is not run some more generations
#Type '''sump burnin=250''', where 250 is the same parameter as above. This will output both a cladogram and a tree file
#Transfer all files into another directory for saving.
