Changes

#*Express and induce protein in culture under appropriate conditions (normally innoculate the previous day):#grow an overnight culture in ~25 mL LB/Amp (+with Chloramphenicol if neededusing Rosetta cells) from a colony <2 weeks post transformation.#add 5 mL overnight culture to 1L TB/Amp and grow at 37C#grow to OD600 of 0.6-1.0 and induce with 10 100 uM IPTG (optimize the concentration of IPTG and duration of induction should be optimized for each protein)

#centrifuge cells 10 min at 9000 RPM to pellet bacteria freeze if stopping at this point.