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==Materials==
*'''S2 cells''' (available as frozen stocks from the Drosophila Genomics Resource Center or vendors)
*'''Sf-900 II SFM medium''' (Invitrogen, cat. no. 10902)- stocked downstairs*'''Penicillin/streptomycin''' 100X 50X mix (Invitrogen, cat. no. 15240062)- stocked downstairs*'''Insect Cell Media'''. Filter together Media (500 mL) and Pen/Strep (5 10 mL) into a tissue culture bottle
==Protocol==
