930
edits
Changes
→Materials: Added product numbers for some materials
==Materials==
*Protein Markers, we use SeaBlue Plus2 from Invitrogen (cat #LC5925)
*Nitrocellulose, we use BioRad 0.45 um membrane from a roll (cat #1620115) and cut to the size of a gel
*Filter Paper, we use BioRad extra thick paper (cat 1703966)
*Transfer Buffer (200 mL Methanol, 100 mL 10X [[ Transfer Buffer ]] to final 1L volume)
*Transfer Apparatus, either Bio-Rad or Invitrogen