930
edits
Changes
→Addition of the reagents: added break for overnight
* Using the multichannel pipet add 50 µl Cortisol ELISA Monoclonal Antibody to each well except the TA, NSB and the blank wells, within 15 min of addition of the tracer.
* Cover each plate with a 96-Well Cover Sheet (Item No. 400012) and incubate overnight at 4°C.
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* Reconstitute Ellman’s Reagent (Item No. 400050) immediately before use. Reconstitute 100 dtn vial with 20 ml of ultrapure water. Reconstitute 250 dtn vial with 50 ml of ultrapure water.
* Empty the wells and rinse five times with ~300 µl Wash Buffer (1X).
