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== Materials ==
* OCT
* Methylbutane or Isopentane (the same thing)
* Liquid Nitrogen or Dry ice (liquid nitrogen preferred)
== Procedures ==
* Place metal beaker of isopentane in container of liquid nitogen.
* Label small pieces of popsicle sticks with mouse ids and add a drop of OCT
* Label ~5 cm<sup>2</sup> pieces of aluminum foil with the mouse IDs
* Rapidly dissect muscle and place on the drop of OCT. Drop the entire OCT/popsicle stick into the cold isopentane. They should stay together, but if they separate take it out and proceed to the next step
* Using tweezers carefully remove the frozen muscle/OCT/wood and flip over the tin foil so the mouse id is on the outsize
* Wrap up the tin foil loosely just to hold the tissue and place back in liquid nitrogen. When complete, take the tin foil out and place in a box and store at -80 until ready to section
* OCT
* Methylbutane or Isopentane (the same thing)
* Liquid Nitrogen or Dry ice (liquid nitrogen preferred)
== Procedures ==
* Place metal beaker of isopentane in container of liquid nitogen.
* Label small pieces of popsicle sticks with mouse ids and add a drop of OCT
* Label ~5 cm<sup>2</sup> pieces of aluminum foil with the mouse IDs
* Rapidly dissect muscle and place on the drop of OCT. Drop the entire OCT/popsicle stick into the cold isopentane. They should stay together, but if they separate take it out and proceed to the next step
* Using tweezers carefully remove the frozen muscle/OCT/wood and flip over the tin foil so the mouse id is on the outsize
* Wrap up the tin foil loosely just to hold the tissue and place back in liquid nitrogen. When complete, take the tin foil out and place in a box and store at -80 until ready to section
