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==Materials==
*Insulin ELISA (Ultra Sensitive Mouse Insulin ELISA Kit from Crystal Chem catalog # 90080)
*Heparinzed capilaries
==Protocol==
#Collect about 2/3 of a capillary of blood.
#Transfer blood to labelled eppendorf tubes.
#Let blood clot
#Centrifuge 20 min at 5000g (about 2000 RPM in eppendorf 5415C centrifuge)
*Insulin ELISA (Ultra Sensitive Mouse Insulin ELISA Kit from Crystal Chem catalog # 90080)
*Heparinzed capilaries
==Protocol==
#Collect about 2/3 of a capillary of blood.
#Transfer blood to labelled eppendorf tubes.
#Let blood clot
#Centrifuge 20 min at 5000g (about 2000 RPM in eppendorf 5415C centrifuge)