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QPCR
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===Run Protocol===
* Set up run using [[Set up qPCR Run on Thermo Cloud and QuantStudio|Thermo Cloud/QuantStudio]] or [[Set up qPCR Run on Roche LightCycler|Roche LightCycler]]
Briefly, log into thermo cloud
*Select "Design and Analysis New, qPCR" from the home screen
*Select "set up plate"
*Select "comparative CT-SYBR"
*Correct volume to read, "10.0uL" instead of "20.0uL"
*Select "Plate set up"
*Navigate to the drop down menu marked "passive reference" and select "none."
*Then import plate setup with linked CSV (MUST be a csv file).
*Save to the cloud and upload on the machine when you insert the plate.
==Calculations==
