52
edits
Changes
no edit summary
* Centrifuge the 1.5mL tube at room temperature (24 degrees Celsius) for 5 minutes at 500 rcf/gauge.
* Once centrifuging is done, the upper fat layer will be evident along with the chunky SVF lower layer as the pellet. The in-between layer solution is called the internatant.
* Cut a 200mL pipette tip’s tip and pipette out the fat layer into a clean 1.5mL tube. Record the volume removed and then split the fat equally into 2 1.5mL tubes. For example, if you remove 800uL of fat (with internatant), then you will end up with 2 1.5mL tubes that have 400uL fat each. Put the fat tubes on ice.
Note: It is hard to isolate the fat while pipetting, so you should anticipate to pipette out some internatant with the fat.
* Using a pipette, aspirate and dump the remaining internatant solution. Be careful not to pipette out the SVF pellet.
