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==Protocol==
Use the following volumes per reaction
Buffer: 4 uL of 5X Go-Taq buffer ("Molecular Biology Stuff" box in freezer)
Forward Primer: .4ul
Reverse Primer: .4ul
dNTPs: .4uL of 2 mM ("Molecular Biology Stuff" box in freezer)
Sterile water: 13.6 uL
Polymerase Go-Taq: 1 uL (6th floor in Genotype Yellow Box in freezer)
Template: 1 uL
see [[Preparing an Agarose Gel]] for details on preparing a DNA gel
