==Protocol==
*#Transfect cells with reporter and pRL vector (50:1) and plate in a 96 well white plate*#Treat cells as required*#Prepare 1X PLB using 5X stock and water*#Wash wells once with 100 ul D-PBS -/-*#Add 20 uL PLB to well and incubate on a shaker for 15 min at RT*#Prepare Stop and Glo Reagent (dilute from 50X in Stop and Glo Buffer). Need 100 uL per assay. Reagent and LARII should be at room temperature*#Set plate reader to luminesence*#Ensure correct measurement head is installed (one light tube) and it is set to do a top read*#Set temperature control to off*#Set plate layout under protocol button for both Luciferase Assay I and Luciferase Assay II*#Add 100 uL of LARII to lysates and read luminescence (2s delay, 10s measurement using Luciferase Assay I*#Add 100 uL of Stop and Glo Reagent and read luminescence again using Luciferase Assay II*#Calculate relative luciferase activity by dividing results from Assay I by Assay II
