Changes

Immunofluoresence

39 bytes added, 17:18, 2 June 2009

→‎Protocol

#Wash once with PBS

#Permeabilize for 10 min with Triton X-100 (0.1% in PBS)

#Wash three times with PBS(5 min each)

#Block for 1-2h with 200 uL of BSA/Ovalbumin (1% of each in PBS) blocking solution

#Incubate overnight with primary antibody in blocking solution

#Wash coverslips 3 times 10 minutes with PBS(5 min each)

#Incubate in 500X secondary solution

#Wash coverslips 3 times 10 minutes with PBS(5 min each)

#Add 10 uL vectashield to glass slide and place cells on slide. Fix with nail polish

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