915
edits
Changes
→Protocol
#Wash once with PBS
#Permeabilize for 10 min with Triton X-100 (0.1% in PBS)
#Wash three times with PBS(5 min each)
#Block for 1-2h with 200 uL of BSA/Ovalbumin (1% of each in PBS) blocking solution
#Incubate overnight with primary antibody in blocking solution
#Wash coverslips 3 times 10 minutes with PBS(5 min each)
#Incubate in 500X secondary solution
#Wash coverslips 3 times 10 minutes with PBS(5 min each)
#Add 10 uL vectashield to glass slide and place cells on slide. Fix with nail polish
