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*echoMRI if the mice differ in body fat levels (see below)
*Syringes
*1.5 mg/mL bOHB in PBS (make as 15mg 15g bOHB in 10mL of PBS, sterile filtered). This will correspond to 1 U/kg injections. If you are using a higher or lower dose of bOHB, add more or less to the 10 mL of PBS, so that injections are 10 uL/g of mass.
*This may need to be adjusted depending on the ketone sensitivity of the mice, and this is based on PGC-1a on chow.
*In general you want the bOHB to increase blood bOHB levels by about 10 to 12-fold (bringing bOHB levels form around .5mmol/L up to the 5 to 6 mmol/L range in the most responsive of your two groups. If your response is lower or higher, you will probably have to change your dose and retry.
