2
edits
Changes
included instructions to make more DMEM
==Materials==
*Media (FBS or NCS as required): Prepare by filtering together 5 mL PSG, 50 mL COS-FBS and one bottle of DMEM
(To prepare DMEM:
1 bottle of DMEM found in 4c in cell culture room
1 tube FBS found in -80 in cell culture room
5 mLs PSG found in door of 4c in cell culture room
500 mL bottle taken from lab
filter found on metal rack behind fume hood
•pour all ingredients into DMEM bottle
•screw filter onto 500 mL bottle
•attach hose onto arm of filter
•pour mixture into filter)
*PBS -/-
*0.05% Trypsin
