12
edits
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Protocol
* Thaw vile of C2C12 cells in 37C water bath approx. 1 min
* Sterilize vile and media bottle with 70%ethanol* Add Pipette 10mL of media to plate in sterile hood
* Pipette cells from vile into plate with media (evenly pipette/distribute throughout plate)
* Lightly swirl the plate until bottom of plate is covered
* Review cells under microscope
* Incubate plate in 37C incubator
