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Preparing Cell Lysates

123 bytes added, 21:25, 26 May 2009
edited phosphatase inhibitors
===RIPA Buffer (for 10mL lysis buffer)===
{| border = "1" cellspacing = "0" cellpadding = "5"! || Final Concentration || per 10 mL || Stock|-|Tris pH7.4 !! || 50mM !! || 500uL|| 1M|-|Na Deoxycholate !! || 0.25% !! || 250uL|| 10%|-| NP-40 !! || 1% !! || 1mL|| 10%|-|NaCl !! || 150mM !! || 375uL|| 4M|-|EDTA !! || 1mM !! || 20uL|| 0.5M|-|NaVO3 !! || 1mM !! || 100uL|| 1M|-|NaF !! 1mM !! 20uL|| 5mM || 100uL || 250mM|-|NaPPi || 25 mM || 1 mL || 500mM|-|-Protease Inhibitors !! 1 tab|}
==Basic Protocol==

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