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#Transfer 400 ul of the bottom layer into a new tube
#Let evaporate overnight at room temperature
#Add'''(50ul)''' of '''Butanol Mixture'''and vortex. See Suggested Volumes for your specific tissue.
#Measure triglyceride levels using SIgma Diagnostic Kit, using 5ul of sample.
##Resuspend triglyceride and glycerol reagent with water if necessary
