Changes
→Plate Preparation
===Plate Preparation===
#Prepare dilutions of primers. Need 9 uL per well. Book 3h on qPCR machine at http://felix2.lsi.umich.edu/ORS/index.php?UID=2fd952b00de25#Prepare primer mix from 5 uM primer pair stocks by adding 0.4 uL primers + 4.6 uL water per reaction
#Get 96 well block and keep on rack. Do not touch bottom of plate.
#Add 1 5 uL template per well. #Add 9 5 uL primer per well
#Using PCR strip and multichannel pipettor, add 10 uL Master mix to each well
