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Insulin Tolerance Test

2 bytes added, 13:22, 24 June 2016
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*Scale
*Beaker for weighing mice
*echoMRI if the mice differ in body fat levels (see [[Taking into Account Differences in Fat Mass#Taking_into_Account_Differences_in _Fat_Mass]])
*Syringes
*0.1 U/mL humulin in PBS (make as 10uL of 100 U/mL in 10 mL, sterile filtered). This will correspond to 1 U/kg injections. If you are using a higher or lower dose of insulin, add more or less to the 10 mL of PBS, so that injections are 10 uL/g of mass.

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