24
edits
Changes
m
no edit summary
##Optional additional cleaning step: Transfer fibers in incubation medium to 5 mL tubes & allow to settle via gravity sedimentation (approx. 20 min). Carefully remove medium & replace with fresh incubation medium. Repeat once more, before placing cleaned fibers into fresh culture dishes.
#Let muscle fibers recover overnight. They are now ready for use experimentally.
==References==
This protocol is a modified method of:
*Cho et al., (2016) FASEB J 30(2):674-87 http://www.fasebj.org/content/30/2/674.full
*Spangenburg et al. http://www.seahorsebio.com/resources/tech-writing/techbrief-intact-muscle-fiber.pdf
