Buchnerd
→Fractionation
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Davebridges
modified volumes to reflect whether using optiprep or not.
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no edit summary
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→References
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-12
→Optiprep Gradient
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Created page with '==Materials== *PBS *Lysis Buffer (25 mM HEPES, 250 mM Sucrose, PI tablet) *Optiprep ==Fractionation== #Wash cells twice with ice cold PBS -/- #Scrape 150 mm dish into 4 mL of Ly...'
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