Preparing an Agarose Gel: Difference between revisions
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[[ SOP - Ethidium Bromide ]] | |||
==Materials== | ==Materials== | ||
*Agarose | *Agarose | ||
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*Thermo Apparatus (Mold, Holder, Comb and Gel Rig) | *Thermo Apparatus (Mold, Holder, Comb and Gel Rig) | ||
==Prepare Gel | ==Prepare Gel== | ||
#Place plastic mold securely in holder | #Place plastic mold securely in holder | ||
#Put well comb in mold | #Put well comb in mold | ||
#Weight out | #Weight out 2.0g agarose and add 100 mL of TAE (2% solution). | ||
#Microwave 90s until dissolved | #Microwave 90s until dissolved | ||
#Add 2 uL Gel Red. Swirl to mix and eliminate sediments | #Add 2 uL Ethidium Bromide or Gel Red. Swirl to mix and eliminate sediments | ||
#Pour into mold | #Pour into mold, add the comb, and wait for it to solidify | ||
#Place gel in cold room if in a hurry | #Place gel in cold room if in a hurry | ||
#After loading, run gel at 100V | #After loading, run gel at 100V | ||
Latest revision as of 12:45, 27 March 2026
Materials
- Agarose
- TAE Buffer (1X): Dilute from 100X stock
- Thermo Apparatus (Mold, Holder, Comb and Gel Rig)
Prepare Gel
- Place plastic mold securely in holder
- Put well comb in mold
- Weight out 2.0g agarose and add 100 mL of TAE (2% solution).
- Microwave 90s until dissolved
- Add 2 uL Ethidium Bromide or Gel Red. Swirl to mix and eliminate sediments
- Pour into mold, add the comb, and wait for it to solidify
- Place gel in cold room if in a hurry
- After loading, run gel at 100V
