Preparing an Agarose Gel

SOP - Ethidium Bromide

Materials

• Agarose
• TAE Buffer (1X): Dilute from 100X stock
• Thermo Apparatus (Mold, Holder, Comb and Gel Rig)

Prepare Gel

1. Place plastic mold securely in holder
2. Put well comb in mold
3. Weight out 2.0g agarose and add 100 mL of TAE (2% solution).
4. Microwave 90s until dissolved
5. Add 2 uL Ethidium Bromide or Gel Red. Swirl to mix and eliminate sediments
6. Pour into mold, add the comb, and wait for it to solidify
7. Place gel in cold room if in a hurry
8. After loading, run gel at 100V