YPD Media and Agar

Revision as of 12:57, 9 October 2009 by Davebridges (Talk | contribs)

Revision as of 12:57, 9 October 2009 by Davebridges (Talk | contribs)

Materials

  • YPD Medium (Clontech 630409)
  • Agar (if making plates)
  • Adenine Hemisulfate if making YPDA. Dissolve to 0.2% and autoclave.

Preparation

  • Weigh out 5g/100 mL of media
  • If making plates, add 2g/100mL of bacto-agar
  • Stir to dissolve and check that pH is at 5.8
  • Autoclave for 15 min at 121C using the liquid cycle
  • If making YPDA plates let cool to ~55C add 1.5 mL/100mL adenine
  • If making rapamycin plates let cool to ~55C add desired amount of rapamycin
  • Pour plates, let dry and store in the cold room