Difference between revisions of "Using the echoMRI"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (wrote initial page) |
Davebridges (Talk | contribs) (Updated after Kari's suggestions) |
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* Make sure the instrument and computer are turned on | * Make sure the instrument and computer are turned on | ||
* Open the software if its not already open | * Open the software if its not already open | ||
| − | * Create a new folder for your experiment in the format YYMMDD-NAMEOFSTUDY | + | * Create a new folder for your experiment in the format '''YYMMDD-<NAMEOFSTUDY>''' |
* Make sure the mouse antenna is connected if you are doing mice, or its taken off if you are doing rats | * Make sure the mouse antenna is connected if you are doing mice, or its taken off if you are doing rats | ||
| − | * Change the selector to Mice or Rats as appropriate | + | * Change the selector to '''Mice''' or '''Rats''' as appropriate |
| − | * Change the water to No (unless you | + | * Change the water stage to '''No''' (unless you need free water determination) |
| − | * Place the canola oil tube in and click '''System Test''' | + | * Place the canola oil tube in and click '''System Test'''. This should take 3-4 minutes. |
==Each Sample== | ==Each Sample== | ||
| − | * Weigh the animal and record the weight. | + | * Weigh the animal and record the weight and animal id. |
* Gently place the mouse/rat in the appropriate sized tube and using the plunger compress it at the end. The animal should be snug but not so tight that it is hurt. If the animal is moving then he is probably not snug enough. | * Gently place the mouse/rat in the appropriate sized tube and using the plunger compress it at the end. The animal should be snug but not so tight that it is hurt. If the animal is moving then he is probably not snug enough. | ||
* Select '''Start Measurement''' or hit '''F5''' and enter the mouse ID, weight and any comments then hit run | * Select '''Start Measurement''' or hit '''F5''' and enter the mouse ID, weight and any comments then hit run | ||
* If you have a lot of animals, then load up the next tube while the first one is being run. | * If you have a lot of animals, then load up the next tube while the first one is being run. | ||
| − | * If you mis-label an animal then click on ''' | + | * If you mis-label an animal then click on '''Review''' then '''Post-Scan Comments''' |
==Exporting Data== | ==Exporting Data== | ||
* Select all your measurements (CTRL-A or shift and point) | * Select all your measurements (CTRL-A or shift and point) | ||
| − | * Go to export table and export it. Change the filename if needed | + | * Go to export table and export it by going to '''Review''' then '''Extract Table'''. Change the filename if needed |
| − | * Copy the entire folder to a USB stick for analysis | + | * Copy the entire folder to a USB stick for analysis including the root folder. Inside that will be a folder with the extracted tables. |
Latest revision as of 15:40, 2 January 2017
Before you start
- Make sure the instrument and computer are turned on
- Open the software if its not already open
- Create a new folder for your experiment in the format YYMMDD-<NAMEOFSTUDY>
- Make sure the mouse antenna is connected if you are doing mice, or its taken off if you are doing rats
- Change the selector to Mice or Rats as appropriate
- Change the water stage to No (unless you need free water determination)
- Place the canola oil tube in and click System Test. This should take 3-4 minutes.
Each Sample
- Weigh the animal and record the weight and animal id.
- Gently place the mouse/rat in the appropriate sized tube and using the plunger compress it at the end. The animal should be snug but not so tight that it is hurt. If the animal is moving then he is probably not snug enough.
- Select Start Measurement or hit F5 and enter the mouse ID, weight and any comments then hit run
- If you have a lot of animals, then load up the next tube while the first one is being run.
- If you mis-label an animal then click on Review then Post-Scan Comments
Exporting Data
- Select all your measurements (CTRL-A or shift and point)
- Go to export table and export it by going to Review then Extract Table. Change the filename if needed
- Copy the entire folder to a USB stick for analysis including the root folder. Inside that will be a folder with the extracted tables.
