Difference between revisions of "TaqMan SNP Assay"
From Bridges Lab Protocols
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*TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X) (Applied Biosystems). | *TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X) (Applied Biosystems). | ||
| − | * | + | *TaqMan® Genotyping Master Mix (supplied at 2X)(Applied Biosystems). |
*StepOnePlus Real-Time PCR System (Applied Biosystems). | *StepOnePlus Real-Time PCR System (Applied Biosystems). | ||
*PC/Laptop with StepOnePlus Software installed. | *PC/Laptop with StepOnePlus Software installed. | ||
| Line 14: | Line 14: | ||
== Protocol == | == Protocol == | ||
| + | # Dilute gDNA samples to 1-5 ng/ul. | ||
| + | # Place gDNA, TaqMan® Genotyping Master Mix, TaqMan® Genotyping Assay on ice. | ||
| + | # Make up Reaction Master mix (see table below). | ||
Revision as of 17:20, 12 November 2015
Materials
- TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X) (Applied Biosystems).
- TaqMan® Genotyping Master Mix (supplied at 2X)(Applied Biosystems).
- StepOnePlus Real-Time PCR System (Applied Biosystems).
- PC/Laptop with StepOnePlus Software installed.
- MicroAmp Fast 96-Well Plates.
- Optical Plate Covers.
- Nuclease-Free Water
- Sample Genomic DNA (extracted as described in Blood DNA Extraction)
- Postive control(s) (Minor allele) (optional, but recommended)
Protocol
- Dilute gDNA samples to 1-5 ng/ul.
- Place gDNA, TaqMan® Genotyping Master Mix, TaqMan® Genotyping Assay on ice.
- Make up Reaction Master mix (see table below).
