Difference between revisions of "TaqMan SNP Assay"
From Bridges Lab Protocols
| Line 9: | Line 9: | ||
*Optical Plate Covers. | *Optical Plate Covers. | ||
*Nuclease-Free Water | *Nuclease-Free Water | ||
| − | + | *Sample Genomic DNA | |
| + | *Postive control(s) (Minor allele) (''optional, but recommended'') | ||
== Protocol == | == Protocol == | ||
Revision as of 16:05, 11 November 2015
Materials
- TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X) (Applied Biosystems).
- TaqMan Genotyping Master Mix (supplied at 2X)(Applied Biosystems).
- StepOnePlus Real-Time PCR System (Applied Biosystems).
- PC/Laptop with StepOnePlus Software installed.
- MicroAmp Fast 96-Well Plates.
- Optical Plate Covers.
- Nuclease-Free Water
- Sample Genomic DNA
- Postive control(s) (Minor allele) (optional, but recommended)
