Difference between revisions of "TaqMan SNP Assay"
From Bridges Lab Protocols
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*TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X) (Applied Biosystems). | *TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X) (Applied Biosystems). | ||
*TaqMan Genotyping Master Mix (supplied at 2X)(Applied Biosystems). | *TaqMan Genotyping Master Mix (supplied at 2X)(Applied Biosystems). | ||
| + | *StepOnePlus Real-Time PCR System (Applied Biosystems). | ||
| + | *PC/Laptop with StepOnePlus Software installed. | ||
*MicroAmp Fast 96-Well Plates. | *MicroAmp Fast 96-Well Plates. | ||
*Optical Plate Covers. | *Optical Plate Covers. | ||
| − | * | + | *Nuclease-Free Water |
| − | + | ||
== Protocol == | == Protocol == | ||
Revision as of 16:01, 11 November 2015
Materials
- TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X) (Applied Biosystems).
- TaqMan Genotyping Master Mix (supplied at 2X)(Applied Biosystems).
- StepOnePlus Real-Time PCR System (Applied Biosystems).
- PC/Laptop with StepOnePlus Software installed.
- MicroAmp Fast 96-Well Plates.
- Optical Plate Covers.
- Nuclease-Free Water
