Difference between revisions of "TaqMan SNP Assay"
From Bridges Lab Protocols
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== Materials == | == Materials == | ||
| − | TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X). | + | TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X) (Applied Biosystems). |
| − | TaqMan Genotyping Master Mix (supplied at 2X). | + | TaqMan Genotyping Master Mix (supplied at 2X)(Applied Biosystems). |
MicroAmp Fast 96-Well Plates. | MicroAmp Fast 96-Well Plates. | ||
Optical Plate Covers. | Optical Plate Covers. | ||
| + | |||
| + | StepOnePlus Real-Time PCR System (Applied Biosystems). | ||
| + | |||
| + | PC/Laptop with StepOnePlus Software installed | ||
== Protocol == | == Protocol == | ||
Revision as of 15:55, 11 November 2015
Materials
TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X) (Applied Biosystems).
TaqMan Genotyping Master Mix (supplied at 2X)(Applied Biosystems).
MicroAmp Fast 96-Well Plates.
Optical Plate Covers.
StepOnePlus Real-Time PCR System (Applied Biosystems).
PC/Laptop with StepOnePlus Software installed
