Difference between revisions of "Set up qPCR Run on Roche LightCycler"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (Copied instructions from qPCR page) |
Davebridges (Talk | contribs) (added categories) |
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| + | [[ Category: RNA ]] | ||
| + | [[ Category: qPCR ]] | ||
| + | [[ Category: Transcription ]] | ||
| + | [[ Category: Expression ]] | ||
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==Run Protocol== | ==Run Protocol== | ||
#Open LightCycler software. | #Open LightCycler software. | ||
Latest revision as of 18:59, 29 January 2017
Run Protocol
- Open LightCycler software.
- Click new experiment.
- Choose SYBR Green option from drop down and make sure that the correct block is in place (it will say 96 or 384).
- Click apply template.
- Go to templates>run templates> bridges SYBR Green 384.
- Load plate and click 'start run'.
- This protocol is set to do the following:
- Activate- 1 cycle @95 degrees for 10s.
- Amplify- 45 cycles @95, 60 and 73 degrees for 15s, 15s and 10s, respectively.
- Melt- 1 cycle @95, 40, 65, 95 and 40 degrees for 1m, 1m, 1s, continuous and 10s, respectively.
