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→Plate Preparation
===Plate Preparation===
#Book 3h on qPCR machine at http://felix2.lsi.umich.edu/ORS
#Prepare primer mix from 5 uM primer pair stocks by adding . Per reaction add 0.4 uL primers + 4.6 uL water per reaction.
#Get 96 well block and keep on rack. Do not touch bottom of plate.
#Add 5 uL template per well.
#Add 5 uL primer per well.
#Add 10 uL per row into 8 wells of a PCR strip plus an extra 10-20 uL
#Using a multichannel pipettor, add 10 uL Master mix to each well
