915
edits
Changes
added 6% gel
*Pour into cassette, overlay with the top layer of water saturated butanol
{| border="1"
! Component || 6% || 7.5% || 10% || 12.5%
|-
| [[SDS-PAGE Separating Gel Buffer]] || 3 mL || 4 3 mL || 5 3 mL || 3 mL
|-
| [[Acrylamide Solution]] || 3 2.4 mL || 3 mL || 3 4 mL || 5 mL
|-
| Water || 6.6 mL|| 6 mL || 5 mL || 4mL
|-
| APS (make fresh, 0.1g/mL in water) || 200uL || 200 uL || 200 uL || 200 uL
|-
| TEMED || 30 uL || 30 uL || 30 uL || 30uL
|}
*For a Phos-Tag Gel, add 24 uL 5 mM Phos-Tag and 24 uL 10 mM MnCl
==Stacking Gel==