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| TEMED || 30 uL || 30 uL || 30 uL || 30uL
|}
*For a Phos-Tag Gel, add 24 uL 5 mM Phos-Tag and 24 uL 10 mM MnClin the separating gel only
==Stacking Gel==
| Water || 4 mL
|-
| APS (make fresh, 0.1g/mL in water) || 200 uL || 200 uL || 200 uL
|-
| TEMED || 30 uL || 30 uL || 30uL
|}
[[Category: SDS-PAGE]]
[[Category: Electrophoresis]]
