Difference between revisions of "Preparation of Tail Samples (for Genotyping)"
From Bridges Lab Protocols
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| − | PBND Solution: Tail Lysis Buffer | + | ==PBND Solution: Tail Lysis Buffer== |
| − | + | * 50mM KCl | |
| − | + | * 10mM Tris HCl (pH~8.3) | |
| − | + | * 0.1 mg/mL MgCl2, 6H20 | |
| − | + | * 0.1 mg/mL Gelatin | |
| − | + | * 0.45% (NP-40) Jgepal | |
| − | + | * 0.45% Tween 20 | |
| − | Protocol | + | ==Protocol== |
| − | + | # Combine 100-200uL of PBND solution with 2ul of Proteinase K and mouse tail in an eppindorf tube or in a well of a 96 well PCR plage | |
| − | + | # Incubate at 55 degrees (O/N) | |
| − | + | # Incubate at 85 degrees for 45 min | |
| − | + | # Run PCR | |
Revision as of 15:06, 27 February 2012
PBND Solution: Tail Lysis Buffer
- 50mM KCl
- 10mM Tris HCl (pH~8.3)
- 0.1 mg/mL MgCl2, 6H20
- 0.1 mg/mL Gelatin
- 0.45% (NP-40) Jgepal
- 0.45% Tween 20
Protocol
- Combine 100-200uL of PBND solution with 2ul of Proteinase K and mouse tail in an eppindorf tube or in a well of a 96 well PCR plage
- Incubate at 55 degrees (O/N)
- Incubate at 85 degrees for 45 min
- Run PCR
