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Preparation of Tail Samples (for Genotyping)

11 bytes added, 13:56, 17 August 2017
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3. Add 37% HCl drop-wise to adjust pH to 8.3.
4. Add ddH20 to 250ml.
 
 
==Protocol==
# Combine 100uL of PBND solution with 2ul of Proteinase K and mouse tail in an eppendorf tube or in a well of a 96 well PCR plate (Proteinase K stock = 10mg/mLin ddH2O)
# Incubate at 55 degrees (16 hours - O/N)
# Incubate at 85 degrees for 60 min
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