PCR Analysis of Tail DNA
see Genotyping Details for strain specific details
Materials
Protocol
Use the following volumes per reaction Buffer: 4 uL of 5X Go-Taq buffer ("Molecular Biology Stuff" box in freezer) Forward Primer: .4ul Reverse Primer: .4ul dNTPs: .4uL of 2 mM ("Molecular Biology Stuff" box in freezer) Sterile water: 13.6 uL Polymerase Go-Taq: 1 uL (6th floor in Genotype Yellow Box in freezer) Template: 1 uL
see Preparing an Agarose Gel for details on preparing a DNA gel