Difference between revisions of "Nr3c1 Genotyping"
From Bridges Lab Protocols
(added cre primers) |
|||
| Line 1: | Line 1: | ||
==PRIMERS== | ==PRIMERS== | ||
| + | nr3c1 | ||
*Fwd: AAACAGGGTTATGCTTGGCA | *Fwd: AAACAGGGTTATGCTTGGCA | ||
*Rev: TGCCTGCTAGGCAAATGATCT | *Rev: TGCCTGCTAGGCAAATGATCT | ||
| − | Make primer dilution at 1uM (10uL each primer + 980uL water). Primers in '''Genotyping Box''' are marked with a star. | + | Cre |
| + | *Fwd: GCATT ACCGG GCAAC GAGTG ATGAG | ||
| + | *Rev: GAGTG AACGA ACCTG GTCGA AATCA GTGCG | ||
| + | |||
| + | |||
| + | Make nr3c1 primer dilution at 1uM (10uL each primer + 980uL water) and Cre at .4uM. Primers in '''Genotyping Box''' are marked with a star. | ||
[[ Category:Genotyping ]] | [[ Category:Genotyping ]] | ||
Revision as of 15:10, 5 June 2020
PRIMERS
nr3c1
- Fwd: AAACAGGGTTATGCTTGGCA
- Rev: TGCCTGCTAGGCAAATGATCT
Cre
- Fwd: GCATT ACCGG GCAAC GAGTG ATGAG
- Rev: GAGTG AACGA ACCTG GTCGA AATCA GTGCG
Make nr3c1 primer dilution at 1uM (10uL each primer + 980uL water) and Cre at .4uM. Primers in Genotyping Box are marked with a star.
