Difference between revisions of "Measuring Fasting Insulin"
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Revision as of 14:46, 16 June 2009
Materials
- Insulin ELISA (Ultra Sensitive Mouse Insulin ELISA Kit from Crystal Chem catalog # 90080)
- Heparinzed capilaries
Protocol
- Collect about 2/3 of a capillary of blood.
- Transfer blood to labelled eppendorf tubes.
- Let blood clot
- Centrifuge 20 min at 5000g (about 2000 RPM in eppendorf 5415C centrifuge)
