Difference between revisions of "Measuring Fasting Insulin"

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(Updated with product details)
 
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==Materials==
 
==Materials==
 
*Insulin ELISA (Ultra Sensitive Mouse Insulin ELISA Kit from Crystal Chem catalog # 90080)
 
*Insulin ELISA (Ultra Sensitive Mouse Insulin ELISA Kit from Crystal Chem catalog # 90080)
*Heparinzed capilaries (Fisher cat# 22-362574)
 
  
 
==Protocol==
 
==Protocol==
#Collect about 2/3 of a capillary of blood, typically via a retro-orbital bleed.
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#Collect serum (see [[Collecting_and_Storing_Mouse_Serum]])
#Transfer blood to labelled eppendorf tubes.
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#Let blood clot for 30 min on ice.
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#Centrifuge 20 min at 2000g (about 5000 RPM in eppendorf 5415C centrifuge).
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#Gentry remove liquid layer and store at -80
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Latest revision as of 15:54, 18 January 2024

Materials

  • Insulin ELISA (Ultra Sensitive Mouse Insulin ELISA Kit from Crystal Chem catalog # 90080)

Protocol

  1. Collect serum (see Collecting_and_Storing_Mouse_Serum)