903
edits
Changes
Added SOP's to front page
See [[Special:Categories]] for as listing of protocols by category.
==Lab Safety==* For notes about required training courses see [[ Safety and Animal Training ]]* For lab-specific standard operating procedures see [[ :Category:SOP]] ==Common Protocols== ===Cell and Tissue Culture===
*[[Splitting Cells]]
*[[Generating DMSO Stocks for Cell Culture]]
*[[Inositol Labeling and Lipid Extraction]]
===Cloning and Molecular Biology===
*[[Transformation of Bacteria]]
*[[Mutagenesis]]
*[[Designing and Generating CRISPR-Cas Mutants]]
===Cell Biology===
*[[Immunofluoresence]]
**[[Colocalization]]
*[[FM4-64 Labeling of Yeast Cells]]
===Protein Analysis===
*[[Preparing a SDS-PAGE Gel]]
*[[Western Blotting]]
*[[Surface Plasmon Resonance - Protein Lipid Interactions]]
====Protein Quantification====
*[[Bradford Assay]]
*[[Quantification by Absorbance at 280nm]]
*[[Using ImageJ to Quantify Bands]]
====Protein Purification====
*[[French Press]]
*[[Purification of GST Fusion Proteins]]
*[[Triglyceride Assay from Drosophila (German Method)]]
===Transcriptional Analysis===
*[[Real Time PCR From Cell Culture]]
*[[Harvesting RNA from Cells grown in monolayer]]
*[[Using Bioconductor To Analyse Microarray Data]]
===Mouse/Fly Protocols===
*[[Maternal Particulate Exposure Breeding]]
===Fly Stocks===
*[[Maintenance of 18C Fly Stocks]]
*[[Fly Food Protocol]]
*[[Monitoring Food Intake]]
===Tissue Preparation===
*[[Harvesting Mouse Tissue]]
*[[Preparation of Protein Lysates from Mouse Tissues]]
*[[Perfusion of Mouse]]
===Media and Buffers=======Yeast Media====
*[[YPD Media and Agar]]
*[[Yeast Selective Media and Agar]]
====Buffers=========Lysis Buffers=====
*[[KRBH Buffer]]
*[[RIPA Buffer]]
*[[CHAPS Lysis Buffer]]
=====SDS-PAGE Buffers=====
*[[SDS-PAGE Separating Gel Buffer]]
*[[SDS-PAGE Stacking Gel Buffer]]
*[[TORC1 Kinase Buffer]]
*[[Acrylamide Solution]]
=====Other Buffers=====
*[[KRBH Buffer]]
*[[MTORC1 Kinase Assay]]
