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Lipofectamine Plasmid Transfection

168 bytes added, 14:18, 30 November 2009
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*Cells in a 6 well dish, plated and at >90% confluence
*Lipofectamine 2000 (Invitrogen cat# 11668-019 for 1.5mL)
 
==Required Amounts==
*Calculate DNA to transfect
*Lipofectamine(in uL) = DNA(in ug) * 2.5
*OptiMEM(in uL, equal for DNA and Lipofectamine) = Lipofectamine(in uL) * 25
==Protocol (6 well dish)==