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KRBH Buffer

455 bytes added, 18:27, 14 October 2009
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{| border="1"
! || Final Concentration || per 500 mL || Stock || Location
|-
|HEPES pH 7.4 || 30mM || 15mL || 1M
|-
|Sodium bicarbonate|| 10mM || 5mL || 1M || Make fresh from powder
|-
|Sodium chloride || 120mM || 15mL || 4M
|-
|Potassium phosphate || 4mM || 2mL || 1M
|-
|Magnesium sulfate|| 1mM || 500uL || 1M
|-
|Calcium chloride || 1mM || 500uL || 1M
|}
*Bring up to 500 mL with ddH20
*Sterile filter buffer and store at 4C
[[ Category:Buffer ]]