Difference between revisions of "Immunoprecipitation"
From Bridges Lab Protocols
(Created page with 'Lyse cells in lysis buffer, such as RIPA buffer with protease inhibitors and phosphatase inhibitors (see link). For each well in a 12 well plate lyse in 1 ml. rotate 0.5 ml lysat...') |
Davebridges (Talk | contribs) m (updated formatting) |
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| − | Lyse cells in lysis buffer, such as RIPA buffer with protease inhibitors and phosphatase inhibitors (see | + | ==Protocol== |
| − | For each well in a 12 well plate lyse in 1 ml. | + | * Lyse cells in lysis buffer, such as RIPA buffer with protease inhibitors and phosphatase inhibitors (see [[RIPA Buffer]]). |
| − | rotate 0.5 ml lysate with 25 ul of protein A/G plus agarose beads (catalog # sc 2003 santa cruz). | + | * For each well in a 12 well plate lyse in 1 ml. |
| + | * rotate 0.5 ml lysate with 25 ul of protein A/G plus agarose beads (catalog # sc 2003 santa cruz). | ||
Revision as of 17:12, 4 April 2012
Protocol
- Lyse cells in lysis buffer, such as RIPA buffer with protease inhibitors and phosphatase inhibitors (see RIPA Buffer).
- For each well in a 12 well plate lyse in 1 ml.
- rotate 0.5 ml lysate with 25 ul of protein A/G plus agarose beads (catalog # sc 2003 santa cruz).
