Changes

Glucose Uptake Assay

11 bytes added, 18:19, 14 October 2009

added link to KRBH Buffer

==Materials==

*2-Deoxyglucose (cold): Sigma D-6134. Dissolve 32.8mg/mL (200 mM) in KRBH

*0.5% BSA in [[KRBHBuffer]]

*Radioactive <sup>14</sup>C-2-deoxyglucose

*Hot 2-DG. Need 50 uL per well, prepare 1 mL KRBH/BSA, 1 uL cold 2-DG and 50 uL radioactive 2-DG

906

edits