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==Materials (Bring Downstairs)==
*Glucometer
*Glucose Test Strips
*Scale
*Beaker for weighing mice
*Syringes
*10% Glucose in PBS (sterile filtered). This will correspond to 1g/kg glucose injections.
==Protocol==
#Remove food from mice for about 6h. Add do not feed tag to cages.
#Bring mice to procedure room.
#Weigh mice, mark tails as necessary and take fasting glucose measurement.
#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL).
#At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse.
:#Immobilize mouse and restrain tail with one hand
:#Aim needle between the midline and the hip bone
:#Insert syringe (do not inject) into cavity
:#Pull up syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over
:#Eject syringe.
*Glucometer
*Glucose Test Strips
*Scale
*Beaker for weighing mice
*Syringes
*10% Glucose in PBS (sterile filtered). This will correspond to 1g/kg glucose injections.
==Protocol==
#Remove food from mice for about 6h. Add do not feed tag to cages.
#Bring mice to procedure room.
#Weigh mice, mark tails as necessary and take fasting glucose measurement.
#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL).
#At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse.
:#Immobilize mouse and restrain tail with one hand
:#Aim needle between the midline and the hip bone
:#Insert syringe (do not inject) into cavity
:#Pull up syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over
:#Eject syringe.