915
edits
Changes
Added details about ipGTT
==SOP==*[[SOP - Needle Capping]]*[[Safety and Animal Training]] ==Materials (Bring Downstairsto Procedure Room)==*Glucometer- AccuChek Advantage*Glucose Test Strips- AccuChek Comfort Curve. Order these through Materiels Service by calling 936-6077 and ordering product 2535. For information see [[https://www31.med.umich.edu/materielsvcs/catalog/details.cfm?stock=2535 here]]. collect in university hospital building, floor B2 in room B2F408 dock 5 across from central pharmacy.
*Scale
*Beaker for weighing mice
*Syringes
*10% Glucose in PBS (make as 1g in 10 mL, sterile filtered). This will correspond to 1g/kg glucose injections.
*If doing an oral glucose tolerance test need a gavage needle
*Timer
==Protocol==
#Remove food from mice for about 6h. Add do not feed tag to cages.
#Bring mice to procedure room. Put water bottle on cage.#Weigh mice, mark tails as necessary and take fasting glucose measurement. If necessary also take blood for [[Measuring Fasting Insulin]] by filling a heparinized capillary about 2/3 full with blood.
#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL).
#At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouseor the mouth if doing an oral glucose tolerance test.:##Immobilize mouse and restrain tail with one hand:##'''For ipGTT''' Aim needle between the midline and the hip bone:##'''For oGTT''' Gently inert gavage needle into mouth##Insert syringe (do not inject) into cavity/mouth:##'''For ipGTT:''' Pull up the syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over:##Eject syringe.#At desired intervals (normally 15, 30, 45, 60, 75, 90, and 120 min), take blood glucose measurements from the tail vein [[Category:Mouse Work]][[Category:Glucose Homeostasis]][[Category:Injections]]