Farnham Lysis Buffer
From Bridges Lab Protocols
RIPA Buffer (for 50mL lysis buffer)
| Final Concentration | per 50 mL | Stock | Location | |
|---|---|---|---|---|
| PIPES pH 8.0 | 5 mM | 250 uL | 1M | 4C |
| KCl | 85 mM | 4.25 mL | 1M | Solutions Shelf |
| NP-40 | 0.5% | 0.357 mL | 70% | Chemical Shelf |
| Protease Inhibitors | 1 mini tablet/1X | 4C |
