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FM4-64 Labeling of Yeast Cells

69 bytes added, 16:26, 20 January 2010
Changed dye concentration to match klionsky protocol
*Instead of FM4-64 we use SynaptoRed (Sigma #S6689). This is resuspended 1mg to 500 uL (2ug/mL) with DMSO and stored in '''Molecular Biology Stuff'''
*Media (see [[YPD Media and Agar]])
*Dye solution. For each cell line need 250 190 uL media + 3 60 uL dye.
==Protocol==
[[Category:Yeast]]
[[Category:Immunoflouresence]]
 
see http://www.mcdb.lsa.umich.edu/labs/klionsky/FM4-64labeling.pdf