162
edits
Changes
→Crosslinking, Lysis and Shearing of DNA
2. Add formaldehyde to a final concentration of 1% directly to the media of adherent cells growing on tissue culture plates, swirl gently, and
incubate at room temperature for 10 minutes (If using 10cm dishes add 0.5mL of the 2.5M glycine stock solution).
3. Stop the cross-linking reaction by adding glycine to a final concentration of 0.125M (If using 10cm dishes add 0.5mL of the 2.5M glycine stock solution) and swirl gently to mix.
4. Remove media from plates and wash cells with equal volume cold (4°C) 1X PBS.