903
edits
Changes
updated cesium concentration
#Load into two ultracentrifuge tubes then add 25 uL EtBR to each tube
#Centrifuge 60 000 RPM (250 000g) O/N in NVT90 rotor
#Withdraw the upper band with a 18g needle and reload into 0.1g/L mL CsCl for second spin
#Remove band and extract EtBr with butanol until clear
#Dialyse overnight in 2L of TE
#Precipitate with 3 vol absolute ethanol and 1/10 vol of 3M Acetate pH 7
#Resuspend to a final concentration of 5-10 mg/mL in TE
